The Molecular Basis of Imidazolinone Herbicide Resistance in Arabidopsis Thaliana Var. Columbia
نویسنده
چکیده
37 Introduction 38 Materials and methods 40 Results 45 Discussion 56 Literature cited 60 IV. SUMMARY AND CONCLUSION 63
منابع مشابه
Molecular Basis of Imidazolinone Herbicide Resistance in Arabidopsis thaliana var Columbia.
Acetolactate synthase (ALS), the first enzyme in the biosynthetic pathway of leucine, isoleucine, and valine, is inhibited by imidazolinone herbicides. To understand the molecular basis of imidazolinone resistance, we isolated the ALS gene from an imazapyr-resistant mutant GH90 of Arabidopsis thaliana. DNA sequence analysis of the mutant ALS gene demonstrated a single-point mutation from G to A...
متن کاملNucleotide sequence of a mutant acetolactate synthase gene from an imidazolinone-resistant Arabidopsis thaliana var. Columbia.
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Ethylmethanesulfonate saturation mutagenesis in Arabidopsis to determine frequency of herbicide resistance.
Plant resistance to glyphosate has been reported far less frequently than resistance to sulfonylurea and imidazolinone herbicides. However, these studies tend to be anecdotal, without side by side comparisons for a single species or natural isolate. In this study, we tested the frequencies of resistance of three herbicides in a controlled ethylmethanesulfonate (EMS) saturation mutagenesis exper...
متن کاملA Mutation Causing Imidazolinone Resistance Maps to the Csr1 Locus of Arabidopsis thaliana.
A mutant of Arabidopsis thaliana, two hundred times more resistant to the imidazolinone herbicide imazapyr than wild-type plants, was isolated by direct selection of seedlings from a mutagenized population. Genetic analysis showed that resistance is due to a single dominant nuclear mutation that could not be separated by recombination from a mutation in the CSR1 gene encoding acetohydroxy acid ...
متن کاملA Mutation Causing Imidazolinone Resistance Maps
A mutant of Arabidopsis thaliana, two hundred times more resistant to the imidazolinone herbicide imazapyr than wild-type plants, was isolated by direct selection of seedlings from a mutagenized population. Genetic analysis showed that resistance is due to a single dominant nuclear mutation that could not be separated by recombination from a mutation in the CSR1 gene encoding acetohydroxy acid ...
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